Importance Of Cell Biology - 1122 Words

For almost a year and a half now, I have entered a more intensive research environment by working as research associate in the LaBarge Lab with the intention of working towards curing breast cancer. My regular routine runs the gamut of activities expected in a cell biology lab: feeding, passaging, expanding, and freezing cell strains grown in our lab; FACS sorting cells based on lineage specific surface markers; immunofluorescence imaging; gene expression analysis. As a research associate, I also make stock solutions and provide support for the staff scientists, post-docs, and graduate students that work in the lab. I work to help them find the answers to the questions they wish to ask about the biological system that is the human breast,†¦show more content†¦And, just as importantly, I experienced the disappointment of failure. A multi-day assay shows equal activity for the control and the experimental samples, FACS sorting collects populations from a control and experiment al condition that have the same gene expression, or the repeat of an experiment shows a different activity result in the samples intended to be biological replicates, essentially calling for another repeat with better experimental technique or a rehash of experimental design. But, after each failure, I observed the ebb of active bench time during a re-evaluation of experimental approach, and a great flow forward of activity as soon as a new approach was determined, showing that failure should inspire new design and ideas rather than true dismay. Currently, I am helping with two projects that involve investigating the effects of a cell’s microenvironment on its phenotypic and molecular characteristics and how these changes relate to the greater onset of breast cancer in older women. One project examines the effect of stiffness on a cell’s regulation of telomerase, a key step in progressing towards cancer. We are focusing on uncovering the actual molecular link involved in this regulation, possibly uncovering a new putative driver of cancer progression. Relation of substrata andShow MoreRelatedThe Importance Of Cells In Biology1000 Words   |  4 Pagesprocesses. Genome variation includes somatic and inherited mutations,Each human cell acts as a tiny but way more complicated computer, but a specific understanding of the biological properties underneath its properties is still lacking. or kill it unbelievable 4 of the that are mentioned steps . Coming together next gen sequencing with advancing mass spect rometry-based proteomics and phosphoproteomics , living cells imaging technologies and 3d genome mapping now provide powerful strategiesRead MoreThe Effect Of Data Analysis On The Importance Of Cells In Biology853 Words   |  4 Pagesformation was marginally reduced after NA supplementation and can be shown in fig 18 suggesting a slightly better preservation on overall genomic stability. The difference in those two cells is because of NAD+ level available in stimulated phase, which overall constrains the repair activity of proliferating cells. So based on the overall data analysis further usage of NA could be conceivable as we showed in the application of lower concentration of NA of 15  µM NAD+ precursor has a positive effectRead MoreWhy We Study the Cell and Its Components.738 Words   |  3 PagesWhy We Study the Cell and Its Components The cell is the most basic form of life essentially. Anything that is living fundamentally is composed of cells. We study the cell because it is the simplest unit of all living organisms and to begin understanding life, we must realize the cell’s structure, whether it is about the different types of cells or how the cell functions. Biology is the study of life and for the human species; the cell is the most basic principle of life, which is why it is soRead MoreScientific Method and Media Piece Article Essay715 Words   |  3 Pagesexperiments. The book â€Å"Campbell Essential Biology with Physiology† discusses how athletes today are using certain types of steroids to enhance their performance. However, do the steroids alone give them super abilities? Or is it a combination of both god given abilities and the steroids? This in my opinion is one of the most interesting scientific topics of today. After reading â€Å"Campbell Essential Biology with Physiology† it helped me understand how science/biology could explain this theory or hypothesesRead MoreCell Migration And Its Importance883 Words   |  4 Pagesfocus question: Cell migration is the movement of cells in a specific direction in order to perform a specific task. Cell migration is necessary in maintaining a healthy organism. In spite of this what type of outside factors prevent, slow down, increase, or enhance cell migration and why? How does this affect the organism overall? Introduction: Provide background information on cell migration and its importance. Then I will discuss the different factors that have an effect on cell migration. I willRead MoreModern Medicine And Technology As A Research Assistant At The Piano Laboratory992 Words   |  4 Pagesovercoming the limitations of contemporary medicine and technology, medicine represents the connection between the applications of research towards patient care. Working as a Research Assistant at the Piano Laboratory, I have an interest in developmental biology. At the Piano Lab, I assist my mentor, Patricia Giselle Cipriani, and her project on the usage of RNAi on ovary-expressed genes and of time-lapse microscopy in order to better understand a comprehensive molecular description of the genetic and evolutionaryRead MoreTechnology : The Promise, Peril, And New Business Of Engineering Life1681 Words   |  7 Pagesridiculous. However, this has all happened as science fiction has turned into reality. We are at a similar point with synthetic biology today as the field is poised to revolutionize the world in ways that we don’t seem to understand yet. Robert Carlson, a principal at Biodesic and a bioengineer, has attempted to describe this concept nearly ten years ago in a book called Biology Is Technology: The Promise, Peril, and New Business of Engineering Life. He is often referred to as the â€Å"authoritative trackerRead MoreA Rese arch Study On Synthetic Biology1324 Words   |  6 Pages2.1 Fundamental research Synthetic biology has a lot to offer in fundamental research. Starting from the most well characterised laboratory organism, E.coli, we are still in half way from full knowledge of understanding the function of all its genes as the functions of 20% of them are still to be identified (Keseler et al., 2011). Moreover there are many more other microorganisms with great importance which are even less-well understood. Synthetic biology provides the ability to make specificRead MoreImportance Of Metabolite Concentration In Cells1147 Words   |  5 PagesMetabolite concentration in cells. The formation of metabolites critically depends on enzymes that act on a parent structure. As such, they represent defined chemical intermediates in a pathway that is designed to modify the parent compound. Because they are intermediates, metabolites tend to be present in small amounts, and for limited and variable periods of time. The importance of metabolites in drug development cannot be overstated. When an organism’s biological systems are disturbed by diseaseRead MoreThe Effects of Varying, Extreme Temperatures and Varying Organic Solvents on a Beet Root Cell Membrane 558 Words   |  3 Pages One of the main structures of a biotic cell is a cell membrane which is produced from a phospholipid (Reece et al., 2011). When a huge number of phospholipids, each comprising a hydrophilic head and a hydrophobi c tail, gather, they rearrange into what is known as a Fluid Mosaic plasma membrane (Reece et al., 2011). This membrane is always in motion and the mosaic created is due to the proteins within the membrane (Reece et al., 2011). Additionally, the membrane is also selectively permeable which